Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay

Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development

Abstract

Uruguayan Creole cattle is taking down to breeds introduced in the American continent around the XVI century. The cytogenetic characterization has showed: a) absence of Y acrocentric chromosome of afroasiatic origin, so there was no introgresion of cebuine breeds; b) robertsonian translocation (robl;29) with an incidence of 4%. It is considered an important rearrangement in chromosome evolution of the Bovidae family, that it is presented in ancestral breeds. Loss of a determinate DNA alphoid sequences (1) rich in CG was found during the evolution of the monocentric translocation. It is proposed to search CCGG sequences among cyto-molecular and genomic level in Creole normal and heterocygous from 1;29, to advance in the study of its chromatin. The restriction enzyme RE Mspl (0,3U/ul) was used to digest chromosome DNA. After this, CBG banding and stainning with propidium iodide were used to take a differential stain of the centromeric chromatin of the 1;29 in front of its homologous and the rest of the chromosomes. A particular chromatin configuration is showed. A light fluorescence is also shown in the 30 pairs of chromosome. The same restriction enzyme is used to digest the genomic DNA of a female heterozygous of robl;29 and a pool of fifteen DNA selected to their high variability and normal karyotype. The samples are run in polyacrilamide desnaturalized gel (6%), and differential bands were stained with silver nitrate. The heterozygous female expressed 43% of alike bands with the population sample, but not own bands is shown. Possible reorganization of chromatin connected wuth evolutive divergents is proposed.