Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus

Authors

  • R. Bove DILAVE Regional Tacuarembó, Ruta 5 km 386, Tacuarembó, Uruguay.
  • F. López DILAVE Regional Tacuarembó, Ruta 5 km 386, Tacuarembó, Uruguay.
  • C. Perera DILAVE Regional Tacuarembó, Ruta 5 km 386, Tacuarembó, Uruguay.
  • B. Carracelas Programa Nacional Carne y Lana, INIA Tacuarembó.
  • D. Torres-Dini Programa Nacional Forestal, INIA Tacuarembó
  • G. De Souza Programa Nacional Carne y Lana, INIA Tacuarembó.
  • C. Azambuja Laboratorio Genia, Montevideo, Uruguay.
  • J. Bermúdez Departamento de Microbiología, Facultad de Veterinaria, UdelaR, Uruguay.
  • F. Alzugaray Departamento de Microbiología, Facultad de Veterinaria, UdelaR, Uruguay.
  • A. Mederos Programa Nacional Carne y Lana, INIA Tacuarembó.

Keywords:

Bovine, Campylobacter fetus venerealis, Abortion, PCR

Abstract

The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-value with a 99% identity, confirming subspecies venerealis.

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Published

2013-12-01

How to Cite

Bove, R., López, F., Perera, C., Carracelas, B., Torres-Dini, D., De Souza, G., … Mederos, A. (2013). Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus. Veterinaria (Montevideo), 49(192), 20–28. Retrieved from https://revistasmvu.com.uy/index.php/smvu/article/view/245

Issue

Vol. 49 No. 192 (2013): December

Section

Technical Notes

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