Use of Ethanol Extracts of Schinus longifolius (Molle) and Eucalyptus grandis (Eucalipto) to Modulate the in vitro Fermentation and Protein Degradation Estimated by the Concentration of Ammonia N

Abstract

The aim of this study was to evaluate the effect of the addition of ethanol extracts of Molle (Schinus longifolius, MO) and Eucalyptus (Eucalyptus grandis, EU) on ruminal fermentation in vitro. We used a 3 × 6 factorial arrangement, combining the effects of inclusion two extracts (dose 1000 mg/mL) and control without extract added on six protein concentrates (soybean meal, sunflower meal 34% and 31% crude protein, canola meal, grains and corn distillers soluble and guar meal) used as substrate. Each of the combinations was incubated in triplicate in rumen fluid. We determined the cumulative gas production at 12 h (PG12) and the pH and ammonia nitrogen concentration (N-NH3) of the liquid at 4 h of incubation. Molle extract decreased the PG12 in sunflower meal and EU extract in canola meal. The pH decreased in two substrates with the addition of MO. Eucalyptus extract did not modify the pH. The N-NH3 concentration decreased with the addition of EU. The ethanol extract of Eucalyptus showed qualities as a modulator of ruminal fermentation, reducing the degradation of nitrogenous substances. The ethanol extract of Molle controlled the fermentation of sunflower meal.