Epigalocatequina-3-galato (EGCG) melhora a maturação in vitro de oócitos ovinos

Autores

  • Andreza Mayara Carneiro Lima Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. Corresponding author: andreza98carneiro@outlook.com https://orcid.org/0000-0001-6192-5937
  • Luana Keally Pimentel de Oliveira Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0001-6440-5515
  • Érika Karoline De Oliveira Aureliano Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0003-3875-2494
  • Damaris Raquel Pires dos Santos Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0005-5269-7983
  • Bruna Moura da Silva Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0003-2832-6689
  • Luanna Mendes Souza Rodrigues Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0002-8994-6339
  • Gabriela Cristina Da Silva Santos Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0003-0876-6054
  • Rafael Soares Dos Anjos Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0002-5979-5229
  • Beatriz Cavalcanti de Freitas Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0002-1073-1555
  • Neilton De Lima Oliveira Junior Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0000-0974-3642
  • Brena Ferreira de Menezes Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0002-3270-9490
  • Jennifer Da Silva Veloso Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0002-8863-1343
  • Pedro Humberto Félix de Souza Department of Technology and Social Sciences, State University of Bahia, 48900-000, Juazeiro-BA, Brazil. https://orcid.org/0009-0004-0218-0743
  • Mabel Freitas Cordeiro Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0003-1963-7311
  • Maria Helena Tavares De Matos Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0001-6215-5292
  • Edilson Soares Lopes Júnior Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0002-4790-8134

DOI:

https://doi.org/10.29155/VET.62.226.6

Palavras-chave:

Antioxidante, Catequina, Ovelha, PIV

Resumo

Este estudo avaliou o efeito da epigalocatequina-3-galato (EGCG) no meio de maturação in vitro (MIV) de oócitos ovinos. Os oócitos foram distribuídos em quatro grupos experimentais submetidos à MIV: CON, composto por meio TCM-199 suplementado com 500 UI de penicilina, 0,5 mg de estreptomicina, 1,25 µg de anfotericina B, 0,2 mM de piruvato de sódio, 10 % (v/v) de soro fetal bovino (SFB) e 15 UI/mL de eCG; e os grupos EGCG25, EGCG50 e EGCG100, nos quais os oócitos foram maturados no mesmo meio do grupo CON, suplementado com 25 µM, 50 µM e 100 µM de EGCG, respectivamente. Ao final do período de maturação, 1.053 oócitos foram utilizados para avaliação da expansão das células do cumulus, configuração da cromatina e quantificação de mitocôndrias ativas, espécies reativas de oxigênio (ERO) e níveis de glutationa (GSH). 391 oócitos foram utilizados para fecundação in vitro (FIV) e cultivo in vitro de embriões (CIV). Os resultados mostraram que o grupo EGCG50 apresentou maiores taxas de MII, mitocôndrias ativas e GSH do que os demais grupos, bem como uma taxa de clivagem superior à do grupo CON. Em conclusão, a concentração de 50 µM de EGCG melhora a taxa de maturação dos oócitos e a produção in vitro de embriões ovinos.

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Publicado

2026-10-15

Como Citar

Carneiro Lima, A. M., Pimentel de Oliveira, L. K., De Oliveira Aureliano, Érika K., Pires dos Santos, D. R., Moura da Silva, B., Mendes Souza Rodrigues, L., … Soares Lopes Júnior, E. (2026). Epigalocatequina-3-galato (EGCG) melhora a maturação in vitro de oócitos ovinos. Veterinaria (Montevideo), 62(226), e20266222606. https://doi.org/10.29155/VET.62.226.6

Edição

v. 62 n. 226 (2026): Julio-Diciembre

Seção

Artigos originais

Licença

Copyright (c) 2026 Sociedad de Medicina Veterinaria del Uruguay-Facultad de Veterinaria, Universidad de la República

Creative Commons License
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.