El epigalocatequina-3-galato (EGCG) mejora la maduración in vitro de los ovocitos de oveja

Autores/as

  • Andreza Mayara Carneiro Lima Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. Corresponding author: andreza98carneiro@outlook.com https://orcid.org/0000-0001-6192-5937
  • Luana Keally Pimentel de Oliveira Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0001-6440-5515
  • Érika Karoline De Oliveira Aureliano Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0003-3875-2494
  • Damaris Raquel Pires dos Santos Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0005-5269-7983
  • Bruna Moura da Silva Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0003-2832-6689
  • Luanna Mendes Souza Rodrigues Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0002-8994-6339
  • Gabriela Cristina Da Silva Santos Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0003-0876-6054
  • Rafael Soares Dos Anjos Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0002-5979-5229
  • Beatriz Cavalcanti de Freitas Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0002-1073-1555
  • Neilton De Lima Oliveira Junior Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0000-0974-3642
  • Brena Ferreira de Menezes Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0002-3270-9490
  • Jennifer Da Silva Veloso Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0009-0002-8863-1343
  • Pedro Humberto Félix de Souza Department of Technology and Social Sciences, State University of Bahia, 48900-000, Juazeiro-BA, Brazil. https://orcid.org/0009-0004-0218-0743
  • Mabel Freitas Cordeiro Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0003-1963-7311
  • Maria Helena Tavares De Matos Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0001-6215-5292
  • Edilson Soares Lopes Júnior Laboratory of Physiology and Biotechnology of Animal Reproduction (LAFIBRA), Federal University of Vale do São Francisco, 56300-000, Campus of Agricultural Sciences, Petrolina-PE, Brazil. https://orcid.org/0000-0002-4790-8134

DOI:

https://doi.org/10.29155/VET.62.226.6

Palabras clave:

Antioxidante, Catequina, Oveja, PIV

Resumen

Este estudio evaluó el efecto de la epigalocatequina-3-galato (EGCG) en el medio de maduración in vitro (MIV) de ovocitos ovinos. Los ovocitos fueron asignados al MIV en cuatro grupos experimentales: CON, compuesto por TCM-199 suplementado con 500 UI de penicilina, 0,5 mg de estreptomicina, 1,25 µg de anfotericina B, 0,2 mM de piruvato sódico, 10 % (v/v) de suero fetal bovino (SFB) y 15 UI/ml de EGCG; y los grupos EGCG25, EGCG50 y EGCG100, donde se maduraron en el mismo medio que el grupo CON, suplementado con 25 µM, 50 µM y 100 µM de EGCG, respectivamente. Al final del periodo de maduración, 1053 ovocitos se utilizaron para evaluar la expansión de las células del cúmulo, la configuración de la cromatina y la cuantificación de las mitocondrias activas, las especies reactivas de oxígeno (ROS) y los niveles de glutatión (GSH). Se utilizaron 391 ovocitos para la fertilización in vitro (FIV) y el cultivo de embriones in vitro (CIV). Los resultados mostraron que el grupo EGCG50 presentó mayores tasas de MII, mitocondrias activas y GSH que los demás grupos, así como una mayor tasa de clivaje que el grupo CON. En conclusión, una concentración de 50 µM de EGCG mejora la tasa de maduración de los ovocitos y la producción de embriones ovinos in vitro.

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Publicado

2026-10-15

Cómo citar

Carneiro Lima, A. M., Pimentel de Oliveira, L. K., De Oliveira Aureliano, Érika K., Pires dos Santos, D. R., Moura da Silva, B., Mendes Souza Rodrigues, L., … Soares Lopes Júnior, E. (2026). El epigalocatequina-3-galato (EGCG) mejora la maduración in vitro de los ovocitos de oveja. Veterinaria (Montevideo), 62(226), e20266222606. https://doi.org/10.29155/VET.62.226.6

Número

Vol. 62 Núm. 226 (2026): Julio-Diciembre

Sección

Artículos originales

Licencia

Derechos de autor 2026 Sociedad de Medicina Veterinaria del Uruguay-Facultad de Veterinaria, Universidad de la República

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.