Generation of specific male markers for the determination of bovine sex by PCR

Authors

  • D. Hirigoyen División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo.
  • H. Bruzzone Giovanelli División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo
  • C. Azambuja Unidad de Biotecnología. Estación Experimental "Las Brujas" INIA. Canelones.
  • M. Stoll División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo. Unidad de Biotecnología. Estación Experimental "Las Brujas" INIA. Canelones.

Keywords:

Cattle, DNA amplification, Polymerase chain reaction, Sex determination

Abstract

The prenatal sex determination in farm animals species of interest, is an estrategic goal from a productive point of view. In this work the DNA amplification in vitro technique (PCR) was used to detect male specific markers (sex marker), using the DNA of six Holstein breed bovines. It was posible the detections of male markers DNA amplification using as little as 5 pg of DNA samples. The diagnostic method applied to bovine resulted specific, sensitive, rapid and economic.

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Published

1994-06-01

How to Cite

Hirigoyen, D., Bruzzone Giovanelli, H., Azambuja, C. ., & Stoll, M. (1994). Generation of specific male markers for the determination of bovine sex by PCR. Veterinaria (Montevideo), 29(122), 12–18. Retrieved from https://revistasmvu.com.uy/index.php/smvu/article/view/667

Issue

Vol. 29 No. 122 (1994): April - June

Section

Original Articles

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