Isolation and characterization of canine, equine and feline mesenchymal stem cells in Uruguay

Authors

  • Kevin Yaneselli Área Inmunología, Departamento Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay.
  • Valeria Campbell Área Inmunología, Departamento Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay. Departamento Equinos, Facultad de Veterinaria, Lasplaces 1550, Montevideo, Uruguay.
  • Agustina Algorta Área Inmunología, Departamento Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay. Departamento de Pequeños Animales, Facultad de Veterinaria, Lasplaces 1550, Montevideo, Uruguay.
  • Clara Bonfiglio Área Inmunología, Departamento Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay.
  • Javier Mirazo Departamento Equinos, Facultad de Veterinaria, Lasplaces 1550, Montevideo, Uruguay.
  • Sofía Fernández Área Inmunología, Departamento Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay.
  • Mauro Ríos Área Inmunología, Departamento Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay.
  • Silvia Llambí Departamento Genética y Mejoramiento Animal, Facultad de Veterinaria, Lasplaces 1550, Montevideo, Uruguay.
  • Jacqueline Maisonnave Área Inmunología, Departamento Ciencias Microbiológicas, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay.

Abstract

Mesenchymal stem cells (MSCs) are of great interest in veterinary medicine due to their  potential use in regenerative medicine. The attractive characteristics are the ability to promote tissue regeneration, to immunomodulate and easiness of extraction from adult animals. The objective of the present work was the isolation and in vitro characterization of adipose tissue-derived MSCs (AD-MSCs) of canine, equine and feline species. The AD-MSCs from 5 canines, 4 equines and 6 felines, were isolated. The cells of each species showed the capacity of forming fibroblast colonies, tridifferentiation in vitro and cells were cryopreserved. In conclusion, it was possible to isolate and in vitro characterize AD-MSCs. A bank of cryopreserved animal MSCs was created, for future therapeutic applications in veterinary medicine and other in vitro studies.

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Published

2018-05-01

How to Cite

Yaneselli, K., Campbell, V., Algorta, A., Bonfiglio, C., Mirazo, J., Fernández, S., … Maisonnave, J. (2018). Isolation and characterization of canine, equine and feline mesenchymal stem cells in Uruguay. Veterinaria (Montevideo), 54(209), 14–19. Retrieved from https://revistasmvu.com.uy/index.php/smvu/article/view/82

Issue

Vol. 54 No. 209 (2018): May

Section

Original Articles

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