Comparison of an indirect ELISA in blood serum versus, an indirect milk ELISA for diagnosis of paratuberculosis in dairy cattle

Performed in the province of Buenos Aires, Argentina

Authors

  • Pedro Sebastián Sosa Doctorando Facultad de Ciencias Veterinarias. Universidad Nacional de La Plata. Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE, Chascomús), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • María Fiorella Alvarado Pinedo Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE, Chascomús), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • Leandro Adrián Di Paolo Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE, Chascomús), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • Luis María Peralta Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE, Chascomús), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
  • Gabriel Eduardo Travería Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE, Chascomús), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.

DOI:

https://doi.org/10.29155/VET.57.216.2

Keywords:

Paratuberculosis, Milk ELISA, Diagnosis, Days in lactation

Abstract

The aim of this paper was to compare the operational characteristics of serum ELISA with milk ELISA, for the diagnosis of bovine paratuberculosis. Using ROC analysis, the area under the curves were 0.84 in serum ELISA and 0.88 in milk ELISA, without significant differences (p= 0.30) between both AUC; the sensitivities were 91.7% and 87.5%, the specificities 82.0% and 86.5% for serum ELISA and milk ELISA respectively. In addition, days in lactation and lactation numbers were also included as variables. Days in lactation were analyzed with correlation and simple linear regression, showing a positive relationship (p < 0.05) between serum ELISA and milk ELISA. Lactations numbers were analyzed only in milk ELISA with ANOVA, significant differences (p= 0.016) were evident between 1st groups with 2nd and> 2nd lactations. The preliminary results demonstrate that serum ELISA presents similar operational characteristics to milk ELISA, being milk ELISA a practical and less invasive alternative to sampling.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Published

2021-08-26

How to Cite

Sosa, P. S., Alvarado Pinedo, M. F., Di Paolo, L. A., Peralta, L. M. ., & Travería, G. E. (2021). Comparison of an indirect ELISA in blood serum versus, an indirect milk ELISA for diagnosis of paratuberculosis in dairy cattle : Performed in the province of Buenos Aires, Argentina. Veterinaria (Montevideo), 57(216), e20215721602. https://doi.org/10.29155/VET.57.216.2

Issue

Section

Original Articles