In vitro DNA amplification (PCR)

instrumentation and basic methodology

Authors

  • D. Hirigoyen División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo.
  • H. Bruzzoni Giovanelli División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo.
  • C. Azambuja Departamento de Biotecnología. Estación Experimental "Las Brujas", INIA. Canelones.
  • M. Stoll Departamento de Biotecnología. Estación Experimental "Las Brujas", INIA. Canelones.

Keywords:

Polymerase chain reaction, DNA amplification, Bioquemical techniques, Genes

Abstract

During the short time in which molecular biology exists as a science, several contributions have been made to improve the technical revolution we are attending nowadays. The DNA Polimerase Chain Reaction (PCR) is a technic that allows to identify new genes, to quantify and characterize nucleotid secuences. This paper makes a brief description of a revolutionary method that may improve scientific research and may modify diagnostic areas in a close future. Some of the recent developments, its instrumentation and methodology are established here.

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Published

1992-03-01

How to Cite

Hirigoyen, D., Bruzzoni Giovanelli, H., Azambuja, C., & Stoll, M. . (1992). In vitro DNA amplification (PCR): instrumentation and basic methodology. Veterinaria (Montevideo), 28(115), 13–19. Retrieved from https://revistasmvu.com.uy/index.php/smvu/article/view/738

Issue

Vol. 28 No. 115 (1992): January - March

Section

Review Articles