Direct transfer of frozen bovine embryos in one step

Authors

  • J. Bonnevaux Transgenes. San José, Uruguay.
  • J. Soñora Transgenes. San José, Uruguay.
  • C. Irozoqui Transgenes. San José, Uruguay.
  • G. De Cuadro Instruments de Médécine Vètérinaire. Cassou. France. Cedex.
  • E. Texeira Ejercicio liberal. Uruguay.
  • J. Baptista Ejercicio liberal. Uruguay.

Keywords:

Bovine embryos implantation, Methodology

Abstract

The methodology to simplify the freezing-thawing and implantation method of bovine embryos is described. For freezing, a buffer solution (Dulbecco) with ethylene glycol as cryoprotectant is used, plus the addition of bovine serum albumin at 4% weight/volume. Defrosting is done 5 seconds in the air and then in a water bath at 25ºC for 20 seconds. The implantation is immediately carried out with extreme delicacy given the fragility of the biological material. The authors report 8 pregnancies out of 17 embryos implanted with this system, considering it satisfactory but perfectible.

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Published

1998-03-01

How to Cite

Bonnevaux, J., Soñora, J., Irozoqui, C., De Cuadro, G., Texeira, E. ., & Baptista, J. (1998). Direct transfer of frozen bovine embryos in one step. Veterinaria (Montevideo), 34(137), 13–14. Retrieved from https://revistasmvu.com.uy/index.php/smvu/article/view/511

Issue

Vol. 34 No. 137 (1998): January - March

Section

Of Interest