Mapping of the MC1-R gene responsible for the colour of the coat, and the LYZ gene, which encodes the stomach lysozyme protein in sheep, using the Prins technique

Authors

  • R. Ponz Laboratorio de Citogenética y Genética Molecular. Facultad de Veterinaria. Universidad de Zaragoza. Zaragoza (España).
  • M. V. Arruga Laboratorio de Citogenética y Genética Molecular. Facultad de Veterinaria. Universidad de Zaragoza. Zaragoza (España).

Keywords:

Gene mapping, Coat colour, MC1-R, Fluorescence, PRINS

Abstract

In order to map the gene MC1-R, responsible of the black coat color in certain ovine breeds, we have used th new PRINS (PRImed IN-Situ Labeling) methodology. This technique allows to asigne and locate loci of genes on metaphasic chromosomes by means of the use of nucleotides marked with fluorochromes. The gen MCR-1 was not known in which chromosome was located; until now. For its animal production importance its location gives an important knowledgement in order to study its relation with other genes linked at the same chromosome. In order to ensure the precision of this methodology we mapped the LYZ gene, responsible of stomachal lysozyme, that is located in cr. 3 in sheep. In the present paper, the rapid results obtained by this methodology, in comparison with other ones based in the in situ hybridization is shown.

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Published

2005-04-01

How to Cite

Ponz, R., & Arruga, M. V. (2005). Mapping of the MC1-R gene responsible for the colour of the coat, and the LYZ gene, which encodes the stomach lysozyme protein in sheep, using the Prins technique. Veterinaria (Montevideo), 40(158), 24–25. Retrieved from https://revistasmvu.com.uy/index.php/smvu/article/view/373

Issue

Vol. 40 No. 158 (2005): April - June

Section

Of Interest