Leukocyte and platelet rich fibrin for the treatment of oronasal fistula in canines
DOI:
https://doi.org/10.29155/VET.61.223.8Keywords:
Periodontal disease, Veterinary dentistry, Blood productsAbstract
Platelet-rich fibrin (L-PRF) is a second-generation blood derivative that can be utilized as a biological filler due to the dense fibrin network formed, acting as a scaffold for cells and sustained release of growth factors, aiding in wound healing. In this study, two clinical cases presented at the Odontostomatology Service of the Veterinary School, UDELAR, involving patients with oro-nasal fistula (ONF) were evaluated. The affected tooth was extracted, and ONF closure was achieved using a mucoperiosteal flap and placement of L-PRF in the tooth socket to promote healing. Favorable outcomes were observed during postoperative follow-ups at days 7, 14, and 30, with complete closure and no complications or recurrences in both clinical cases. In these cases, the use of L-PRF placed with the mucoperiosteal flap was able to withstand oral mechanical forces, with no suture dehiscence or ONF recurrence, resulting in satisfactory healing. The use of this regenerative technique appears to offer a new approach for alveolar defect closure, with advantages including its speed, cost-effectiveness, simplicity, ease of implementation in a veterinary clinic, safety, and low risk.
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American Veterinary Dental College. (2024). AVDC® Nomenclature. https://avdc.org/avdc-nomenclature/
Atherton, D. D., & Boorman, J. G. (2016). Use of a purified collagen membrane to aid closure of palatal fistulae. Journal of Plastic, Reconstructive & Aesthetic Surgery, 69(7), 1003-1007. https://doi.org/10.1016/J.BJPS.2016.02.009
Dohan, D. M., Choukroun, J., Diss, A., Dohan, S. L., Dohan, A. J. J., Mouhyi, J., & Gogly, B. (2006). Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 101(3), e37-e44.
Fossum, T. W. (2009). Cirugía en pequeños animales (3.a ed.). Elsevier.
Fujioka‐Kobayashi, M., Miron, R. J., Hernandez, M., Kandalam, U., Zhang, Y., & Choukroun, J. (2017). Optimized Platelet-Rich Fibrin with the Low-Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response. Journal of Periodontology, 88(1), 112-121. https://doi.org/10.1902/jop.2016.160443
Kornsuthisopon, C., Pirarat, N., Osathanon, T., & Kalpravidh, C. (2020). Autologous platelet-rich fibrin stimulates canine periodontal regeneration. Scientific Reports, 10(1). 1850. https://doi.org/10.1038/S41598-020-58732-X
Niemiec, B., Gawor, J., Nemec, A., Clarke, D., McLeod, K., Tutt, C., Gioso, M., Steagall, P. V., Chandler, M., Morgenegg, G., Jouppi, R., & McLeod, K. (2020). World Small Animal Veterinary Association Global Dental Guidelines. The Journal of Small Animal Practice, 61(7), 395-403. https://doi.org/10.1111/JSAP.13113
Salgado-Peralvo, Á. O., Salgado-García, Á., Arriba-Fuente, L., Salgado-Peralvo, Á. O., Salgado-García, Á., & Arriba-Fuente, L. (2017). Nuevas tendencias en regeneración tisular: fibrina rica en plaquetas y leucocitos. Revista Española de Cirugía Oral y Maxilofacial, 39(2), 91-98. https://doi.org/10.1016/J.MAXILO.2016.03.001
Shannon, S. (2022). Surgical Repair of Acquired Oronasal Fistulas Secondary to Periodontal Disease in Dogs Using a Flexible Bone Membrane: A Case Series. Journal of Veterinary Dentistry, 39(1), 63-70. https://doi.org/10.1177/08987564211067862
Soares, C. S., Babo, P. S., Faria, S., Pires, M. A., & Carvalho, P. P. (2021). Standardized Platelet-Rich Fibrin (PRF) from canine and feline origin: An analysis on its secretome pattern and architectural structure. Cytokine, 148, 155695. https://doi.org/10.1016/J.CYTO.2021.155695
Soares, C. S., Barros, L. C., Saraiva, V., Gomez-Florit, M., Babo, P. S., Dias, I. R., Reis, R. L., Carvalho, P. P., & Gomes, M. E. (2018). Bioengineered surgical repair of a chronic oronasal fistula in a cat using autologous platelet-rich fibrin and bone marrow with a tailored 3D printed implant. Journal of Feline Medicine and Surgery, 20(9), 835-843. https://doi.org/10.1177/1098612X18789549
Soukup, J. W., Snyder, C. J., & Gengier, W. R. (2009). Free auricular cartilage autograft for repair of an oronasal fistula in a dog. Journal of Veterinary Dentistry, 26(2), 86-95. https://doi.org/10.1177/089875640902600203
Tambella, A. M., Bartocetti, F., Rossi, G., Galosi, L., Catone, G., Falcone, A., & Vullo, C. (2020). Effects of Autologous Platelet-Rich Fibrin in Post-Extraction Alveolar Sockets: A Randomized, Controlled Split-Mouth Trial in Dogs with Spontaneous Periodontal Disease. Animals, 10(8), 1343. https://doi.org/10.3390/ANI10081343
To, M., Su, C. -Y., Hidaka, K., Okudera, T., & Matsuo, M. (2019). Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation. Anatomical Science International, 94(3), 238-244. https://doi.org/10.1007/S12565-019-00479-1
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